other:Article Title: Metabolic Blockade-Based Genome Mining of Malbranchea circinata SDU050: Discovery of Diverse Secondary Metabolites.
Article Snippet: Bacterial Strains MIC (μg/mL) of Standard Diameters (mm) of Compounds 1, 10–13 Vancomycin Ampicillin 1 10 11 12 13 Staphylococcus aureus ATCC 29213 0.5 1 64 64 >64 >64 >64 Staphylococcus aureus 16339 1 64 >64 32 >64 >64 >64 MRSA 1 64 >64 64 >64 >64 >64 Enterococcus faecalis ATCC 29212 1 1 >64 >64 >64 >64 >64 Bacillus subtilis BS01 0.25 0.25 64 32 >64 >64 >64 Staphylococcus simulans AKA1 1 >64 >64 >64 >64 >64 >64 Enterococcus gallinarum 5F52C 4 8 >64 >64 >64 >64 >64 Staphylococcus epidermidis SRH–sep 8 64 >64 >64 >64 >64 >64 Vancomycin and ampicillin were used as positive controls.
Article Title: Phytochemical Analysis and Specific Activities of Bark and Flower Extracts from Four Magnolia Plant Species
Article Snippet: Bacterial Strains MIC (μg/mL) Ampicillin Gentamicin Tetracycline M. champaca M. denudata M. grandiflora M. officinalis Bark Flo- wer Bark Flower Bark Flower Bark Flo- wer Staphylococcus aureus ATCC 43300(MRSA) n.a.
Control:Article Title: Exploring the antimicrobial and antioxidant properties of TdGASA2 protein: From molecular insights to a promising natural preservative for Tunisian cheese shelf-life enhancement
Article Snippet: Gibberellic acid-stimulated Arabidopsis (GASA) proteins, also known as Snakin proteins, have emerged as key players in plant development and stress tolerance.. The investigation of the role of TdGASA2 in host defense responses revealed dynamic expression patterns under different stress conditions.. TdGASA2 expression showed a consistent increase after wounding, methyl jasmonate (MeJA), and ethylene (ETP) treatments, peaking after 12–24 h for MeJA and ETP and after 1 h for pathogen elicitor (PA) treatment.
Article Title: Unveiling the Antibacterial Efficacy of a Benzonitrile Small Molecule, IITR00210, in Shigella Infection.
Article Snippet: .. Table S1: Minimum inhibitory concentration (MIC) of IITR00210 and Kanamycin (control) against various bacterial strains MIC (μg/ml) Strains (Gram-negative) IITR00210 Kanamycin Escherichia coli ATCC 25922 4 2 Escherichia coli MG1655 8 2 Escherichia coli BW25113 16 2 Salmonella enteritidis 1-2 1 Salmonella typhi (NKN 45) 2 2 Salmonella typhimurium (NKN 12) 4 1 Salmonella Vi positive (NKN 10) 2 2 Salmonella enterica ATCC 10708 (RPT 81) 4 1 Salmonella typhi sero enterica (NKN 5) 1 0.5 Shigella flexneri ATCC 9199 (RPT 80) 2 1 Shigella wild type (NKN 47) 2 4 Vibrio cholerae (NKN 43) 4 8 Cronobacter sakazakii (NKN 7) 4 2 Acinetobacter baumannii ATCC 17978 4 2 Acinetobacter baumannii SDF 4 1 Acinetobacter baumannii MTCC 1425 4 2 Gram-negative clinical isolates S6 Shigella boydii (IDH-00007) 4 4 Shigella boydii (IDH-00148) 8 4 Shigella flexneri 2a (IDH-00009) 4 4 Shigella flexneri 2a (IDH-00149) 4 4 Shigella sonnei (IDH-01529) 4 2 Shigella sonnei (IDH-01541) 4 2 Escherichia coli O157:H7 4 2 Klebsiella pneumoniae ATCC 700603 32 32 Klebsiella pneumoniae U25 MDR 16 64 Acinetobacter baumannii AYE MDR 64 >128 Gram-positive strains Bacillus cereus ATCC 11778 16 2 Staphylococcus aureus ATCC 29213 32 1 Clostridium perfringens (NKN 6) 4 >128 Listeria monocytogenes (NKN 8) 4 >64 Lactobacillus brevis (NKN 55) 256 0.5 Lactobacillus brevis (NKN 52) >256 4 Streptococcus thermophilus 128 32 S7 Table S2: Solubility assay of IITR00210 IITR00210 (2—nitro- benzonitrile)Assay parameters Working stock Assay medium % Solubility Kinetic Solubility 160 μM in DMSO 100 mM PBS (pH 7.4) 99 Thermodynamic solubility 13.5 mM in PBS (pH 7.4) 100 mM PBS (pH 7.4) 30 Table S3: Plasma protein binding of IITR00210 IITR00210 Assay parameters Working stock Assay medium Percentage Recovery of compound Plasma protein binding 3.2 mM in DMSO 50% human blood plasma + 1X PBS (pH7.4) 90 Table S4: Strains used in the study. .. Lab strains Strain/Relevant genotype Source/Reference RPT 67 Escherichia coli ATCC 25922 ATCC, USA RPT 26 Escherichia coli MG1655 Lab collection RPT 315 Escherichia coli BW 25113 Lab collection S8 NKN 4 Escherichia coli O157:H7 Lab collection RPT 71 Acinetobacter baumannii ATCC 17978 ATCC, USA RPT 72 Acinetobacter baumannii SDF (ATCC BAA-1709) Lab collection RPT 57 Acinetobacter baumannii MTCC 1425 MTCC, IMTECH-Chandigarh RPT 73 Acinetobacter baumannii AYE (ATCC BAA-1710) ATCC, USA RPT 80 Shigella flexneri ATCC 9199 ATCC, USA NKN 47 Shigella wild type Lab collection RPT 81 Salmonella enterica ATCC 10708 ATCC, USA NKN 5 Salmonella enterica Serovar Typhimurium Lab collection NKN 45 Salmonella typhi Lab collection NKN 12 Salmonella typhimurium Lab collection NKN 10 Salmonella Vi positive Lab collection NASF-sal2 Salmonella enteritidis Lab collection NKN 43 Vibrio cholerae Lab collection RPTS 3 Shigella boydii (IDH-00007) Clinical isolate, THSTI-India RPTS 2 Shigella boydii (IDH-00148) Clinical isolate, THSTI-India RPTS 1 Shigella flexneri 2a (IDH-00009) Clinical isolate, THSTI-India RPTS 6 Shigella flexneri 2a (IDH-00149) Clinical isolate, THSTI-India RPTS 5 Shigella sonnei (IDH-01529) Clinical isolate, THSTI-India RPTS 4 Shigella sonnei (IDH-01541) Clinical isolate, THSTI-India S9 RPT 76 Staphylococcus aureus ATCC 29213 ATCC, USA RPT 84 Bacillus cereus ATCC 11778 ATCC, USA NKN 8 Listeria monocytogens Lab collection NKN 6 Clostridium perfringens Lab collection NKN 7 Cronobacter sakazakii Lab collection RPT 79 Klebsiella pneumoniae ATCC 600703 ATCC, USA RPT 293 Klebsiella pneumoniae U25 Lab collection NKN 51 Lactobacillus fermentum Lab collection NKN 55 Lactococcus brevis Lab collection NKN 54 Lactobacillus plantarum Lab collection NKN 52 Lactobacillus brevis Lab collection NKN 189 Lactobacillus rhamnosus (L44) Lab collection NKN 147 Lactococcus lactis NZ9000 Lab collection NKN 188 Streptococcus thermophilus NCDC 74 Lab collection Table S5: Plasmids used in the study.
Concentration Assay:Article Title: Unveiling the Antibacterial Efficacy of a Benzonitrile Small Molecule, IITR00210, in Shigella Infection.
Article Snippet: .. Table S1: Minimum inhibitory concentration (MIC) of IITR00210 and Kanamycin (control) against various bacterial strains MIC (μg/ml) Strains (Gram-negative) IITR00210 Kanamycin Escherichia coli ATCC 25922 4 2 Escherichia coli MG1655 8 2 Escherichia coli BW25113 16 2 Salmonella enteritidis 1-2 1 Salmonella typhi (NKN 45) 2 2 Salmonella typhimurium (NKN 12) 4 1 Salmonella Vi positive (NKN 10) 2 2 Salmonella enterica ATCC 10708 (RPT 81) 4 1 Salmonella typhi sero enterica (NKN 5) 1 0.5 Shigella flexneri ATCC 9199 (RPT 80) 2 1 Shigella wild type (NKN 47) 2 4 Vibrio cholerae (NKN 43) 4 8 Cronobacter sakazakii (NKN 7) 4 2 Acinetobacter baumannii ATCC 17978 4 2 Acinetobacter baumannii SDF 4 1 Acinetobacter baumannii MTCC 1425 4 2 Gram-negative clinical isolates S6 Shigella boydii (IDH-00007) 4 4 Shigella boydii (IDH-00148) 8 4 Shigella flexneri 2a (IDH-00009) 4 4 Shigella flexneri 2a (IDH-00149) 4 4 Shigella sonnei (IDH-01529) 4 2 Shigella sonnei (IDH-01541) 4 2 Escherichia coli O157:H7 4 2 Klebsiella pneumoniae ATCC 700603 32 32 Klebsiella pneumoniae U25 MDR 16 64 Acinetobacter baumannii AYE MDR 64 >128 Gram-positive strains Bacillus cereus ATCC 11778 16 2 Staphylococcus aureus ATCC 29213 32 1 Clostridium perfringens (NKN 6) 4 >128 Listeria monocytogenes (NKN 8) 4 >64 Lactobacillus brevis (NKN 55) 256 0.5 Lactobacillus brevis (NKN 52) >256 4 Streptococcus thermophilus 128 32 S7 Table S2: Solubility assay of IITR00210 IITR00210 (2—nitro- benzonitrile)Assay parameters Working stock Assay medium % Solubility Kinetic Solubility 160 μM in DMSO 100 mM PBS (pH 7.4) 99 Thermodynamic solubility 13.5 mM in PBS (pH 7.4) 100 mM PBS (pH 7.4) 30 Table S3: Plasma protein binding of IITR00210 IITR00210 Assay parameters Working stock Assay medium Percentage Recovery of compound Plasma protein binding 3.2 mM in DMSO 50% human blood plasma + 1X PBS (pH7.4) 90 Table S4: Strains used in the study. .. Lab strains Strain/Relevant genotype Source/Reference RPT 67 Escherichia coli ATCC 25922 ATCC, USA RPT 26 Escherichia coli MG1655 Lab collection RPT 315 Escherichia coli BW 25113 Lab collection S8 NKN 4 Escherichia coli O157:H7 Lab collection RPT 71 Acinetobacter baumannii ATCC 17978 ATCC, USA RPT 72 Acinetobacter baumannii SDF (ATCC BAA-1709) Lab collection RPT 57 Acinetobacter baumannii MTCC 1425 MTCC, IMTECH-Chandigarh RPT 73 Acinetobacter baumannii AYE (ATCC BAA-1710) ATCC, USA RPT 80 Shigella flexneri ATCC 9199 ATCC, USA NKN 47 Shigella wild type Lab collection RPT 81 Salmonella enterica ATCC 10708 ATCC, USA NKN 5 Salmonella enterica Serovar Typhimurium Lab collection NKN 45 Salmonella typhi Lab collection NKN 12 Salmonella typhimurium Lab collection NKN 10 Salmonella Vi positive Lab collection NASF-sal2 Salmonella enteritidis Lab collection NKN 43 Vibrio cholerae Lab collection RPTS 3 Shigella boydii (IDH-00007) Clinical isolate, THSTI-India RPTS 2 Shigella boydii (IDH-00148) Clinical isolate, THSTI-India RPTS 1 Shigella flexneri 2a (IDH-00009) Clinical isolate, THSTI-India RPTS 6 Shigella flexneri 2a (IDH-00149) Clinical isolate, THSTI-India RPTS 5 Shigella sonnei (IDH-01529) Clinical isolate, THSTI-India RPTS 4 Shigella sonnei (IDH-01541) Clinical isolate, THSTI-India S9 RPT 76 Staphylococcus aureus ATCC 29213 ATCC, USA RPT 84 Bacillus cereus ATCC 11778 ATCC, USA NKN 8 Listeria monocytogens Lab collection NKN 6 Clostridium perfringens Lab collection NKN 7 Cronobacter sakazakii Lab collection RPT 79 Klebsiella pneumoniae ATCC 600703 ATCC, USA RPT 293 Klebsiella pneumoniae U25 Lab collection NKN 51 Lactobacillus fermentum Lab collection NKN 55 Lactococcus brevis Lab collection NKN 54 Lactobacillus plantarum Lab collection NKN 52 Lactobacillus brevis Lab collection NKN 189 Lactobacillus rhamnosus (L44) Lab collection NKN 147 Lactococcus lactis NZ9000 Lab collection NKN 188 Streptococcus thermophilus NCDC 74 Lab collection Table S5: Plasmids used in the study.
Solubility:Article Title: Unveiling the Antibacterial Efficacy of a Benzonitrile Small Molecule, IITR00210, in Shigella Infection.
Article Snippet: .. Table S1: Minimum inhibitory concentration (MIC) of IITR00210 and Kanamycin (control) against various bacterial strains MIC (μg/ml) Strains (Gram-negative) IITR00210 Kanamycin Escherichia coli ATCC 25922 4 2 Escherichia coli MG1655 8 2 Escherichia coli BW25113 16 2 Salmonella enteritidis 1-2 1 Salmonella typhi (NKN 45) 2 2 Salmonella typhimurium (NKN 12) 4 1 Salmonella Vi positive (NKN 10) 2 2 Salmonella enterica ATCC 10708 (RPT 81) 4 1 Salmonella typhi sero enterica (NKN 5) 1 0.5 Shigella flexneri ATCC 9199 (RPT 80) 2 1 Shigella wild type (NKN 47) 2 4 Vibrio cholerae (NKN 43) 4 8 Cronobacter sakazakii (NKN 7) 4 2 Acinetobacter baumannii ATCC 17978 4 2 Acinetobacter baumannii SDF 4 1 Acinetobacter baumannii MTCC 1425 4 2 Gram-negative clinical isolates S6 Shigella boydii (IDH-00007) 4 4 Shigella boydii (IDH-00148) 8 4 Shigella flexneri 2a (IDH-00009) 4 4 Shigella flexneri 2a (IDH-00149) 4 4 Shigella sonnei (IDH-01529) 4 2 Shigella sonnei (IDH-01541) 4 2 Escherichia coli O157:H7 4 2 Klebsiella pneumoniae ATCC 700603 32 32 Klebsiella pneumoniae U25 MDR 16 64 Acinetobacter baumannii AYE MDR 64 >128 Gram-positive strains Bacillus cereus ATCC 11778 16 2 Staphylococcus aureus ATCC 29213 32 1 Clostridium perfringens (NKN 6) 4 >128 Listeria monocytogenes (NKN 8) 4 >64 Lactobacillus brevis (NKN 55) 256 0.5 Lactobacillus brevis (NKN 52) >256 4 Streptococcus thermophilus 128 32 S7 Table S2: Solubility assay of IITR00210 IITR00210 (2—nitro- benzonitrile)Assay parameters Working stock Assay medium % Solubility Kinetic Solubility 160 μM in DMSO 100 mM PBS (pH 7.4) 99 Thermodynamic solubility 13.5 mM in PBS (pH 7.4) 100 mM PBS (pH 7.4) 30 Table S3: Plasma protein binding of IITR00210 IITR00210 Assay parameters Working stock Assay medium Percentage Recovery of compound Plasma protein binding 3.2 mM in DMSO 50% human blood plasma + 1X PBS (pH7.4) 90 Table S4: Strains used in the study. .. Lab strains Strain/Relevant genotype Source/Reference RPT 67 Escherichia coli ATCC 25922 ATCC, USA RPT 26 Escherichia coli MG1655 Lab collection RPT 315 Escherichia coli BW 25113 Lab collection S8 NKN 4 Escherichia coli O157:H7 Lab collection RPT 71 Acinetobacter baumannii ATCC 17978 ATCC, USA RPT 72 Acinetobacter baumannii SDF (ATCC BAA-1709) Lab collection RPT 57 Acinetobacter baumannii MTCC 1425 MTCC, IMTECH-Chandigarh RPT 73 Acinetobacter baumannii AYE (ATCC BAA-1710) ATCC, USA RPT 80 Shigella flexneri ATCC 9199 ATCC, USA NKN 47 Shigella wild type Lab collection RPT 81 Salmonella enterica ATCC 10708 ATCC, USA NKN 5 Salmonella enterica Serovar Typhimurium Lab collection NKN 45 Salmonella typhi Lab collection NKN 12 Salmonella typhimurium Lab collection NKN 10 Salmonella Vi positive Lab collection NASF-sal2 Salmonella enteritidis Lab collection NKN 43 Vibrio cholerae Lab collection RPTS 3 Shigella boydii (IDH-00007) Clinical isolate, THSTI-India RPTS 2 Shigella boydii (IDH-00148) Clinical isolate, THSTI-India RPTS 1 Shigella flexneri 2a (IDH-00009) Clinical isolate, THSTI-India RPTS 6 Shigella flexneri 2a (IDH-00149) Clinical isolate, THSTI-India RPTS 5 Shigella sonnei (IDH-01529) Clinical isolate, THSTI-India RPTS 4 Shigella sonnei (IDH-01541) Clinical isolate, THSTI-India S9 RPT 76 Staphylococcus aureus ATCC 29213 ATCC, USA RPT 84 Bacillus cereus ATCC 11778 ATCC, USA NKN 8 Listeria monocytogens Lab collection NKN 6 Clostridium perfringens Lab collection NKN 7 Cronobacter sakazakii Lab collection RPT 79 Klebsiella pneumoniae ATCC 600703 ATCC, USA RPT 293 Klebsiella pneumoniae U25 Lab collection NKN 51 Lactobacillus fermentum Lab collection NKN 55 Lactococcus brevis Lab collection NKN 54 Lactobacillus plantarum Lab collection NKN 52 Lactobacillus brevis Lab collection NKN 189 Lactobacillus rhamnosus (L44) Lab collection NKN 147 Lactococcus lactis NZ9000 Lab collection NKN 188 Streptococcus thermophilus NCDC 74 Lab collection Table S5: Plasmids used in the study.
Clinical Proteomics:Article Title: Unveiling the Antibacterial Efficacy of a Benzonitrile Small Molecule, IITR00210, in Shigella Infection.
Article Snippet: .. Table S1: Minimum inhibitory concentration (MIC) of IITR00210 and Kanamycin (control) against various bacterial strains MIC (μg/ml) Strains (Gram-negative) IITR00210 Kanamycin Escherichia coli ATCC 25922 4 2 Escherichia coli MG1655 8 2 Escherichia coli BW25113 16 2 Salmonella enteritidis 1-2 1 Salmonella typhi (NKN 45) 2 2 Salmonella typhimurium (NKN 12) 4 1 Salmonella Vi positive (NKN 10) 2 2 Salmonella enterica ATCC 10708 (RPT 81) 4 1 Salmonella typhi sero enterica (NKN 5) 1 0.5 Shigella flexneri ATCC 9199 (RPT 80) 2 1 Shigella wild type (NKN 47) 2 4 Vibrio cholerae (NKN 43) 4 8 Cronobacter sakazakii (NKN 7) 4 2 Acinetobacter baumannii ATCC 17978 4 2 Acinetobacter baumannii SDF 4 1 Acinetobacter baumannii MTCC 1425 4 2 Gram-negative clinical isolates S6 Shigella boydii (IDH-00007) 4 4 Shigella boydii (IDH-00148) 8 4 Shigella flexneri 2a (IDH-00009) 4 4 Shigella flexneri 2a (IDH-00149) 4 4 Shigella sonnei (IDH-01529) 4 2 Shigella sonnei (IDH-01541) 4 2 Escherichia coli O157:H7 4 2 Klebsiella pneumoniae ATCC 700603 32 32 Klebsiella pneumoniae U25 MDR 16 64 Acinetobacter baumannii AYE MDR 64 >128 Gram-positive strains Bacillus cereus ATCC 11778 16 2 Staphylococcus aureus ATCC 29213 32 1 Clostridium perfringens (NKN 6) 4 >128 Listeria monocytogenes (NKN 8) 4 >64 Lactobacillus brevis (NKN 55) 256 0.5 Lactobacillus brevis (NKN 52) >256 4 Streptococcus thermophilus 128 32 S7 Table S2: Solubility assay of IITR00210 IITR00210 (2—nitro- benzonitrile)Assay parameters Working stock Assay medium % Solubility Kinetic Solubility 160 μM in DMSO 100 mM PBS (pH 7.4) 99 Thermodynamic solubility 13.5 mM in PBS (pH 7.4) 100 mM PBS (pH 7.4) 30 Table S3: Plasma protein binding of IITR00210 IITR00210 Assay parameters Working stock Assay medium Percentage Recovery of compound Plasma protein binding 3.2 mM in DMSO 50% human blood plasma + 1X PBS (pH7.4) 90 Table S4: Strains used in the study. .. Lab strains Strain/Relevant genotype Source/Reference RPT 67 Escherichia coli ATCC 25922 ATCC, USA RPT 26 Escherichia coli MG1655 Lab collection RPT 315 Escherichia coli BW 25113 Lab collection S8 NKN 4 Escherichia coli O157:H7 Lab collection RPT 71 Acinetobacter baumannii ATCC 17978 ATCC, USA RPT 72 Acinetobacter baumannii SDF (ATCC BAA-1709) Lab collection RPT 57 Acinetobacter baumannii MTCC 1425 MTCC, IMTECH-Chandigarh RPT 73 Acinetobacter baumannii AYE (ATCC BAA-1710) ATCC, USA RPT 80 Shigella flexneri ATCC 9199 ATCC, USA NKN 47 Shigella wild type Lab collection RPT 81 Salmonella enterica ATCC 10708 ATCC, USA NKN 5 Salmonella enterica Serovar Typhimurium Lab collection NKN 45 Salmonella typhi Lab collection NKN 12 Salmonella typhimurium Lab collection NKN 10 Salmonella Vi positive Lab collection NASF-sal2 Salmonella enteritidis Lab collection NKN 43 Vibrio cholerae Lab collection RPTS 3 Shigella boydii (IDH-00007) Clinical isolate, THSTI-India RPTS 2 Shigella boydii (IDH-00148) Clinical isolate, THSTI-India RPTS 1 Shigella flexneri 2a (IDH-00009) Clinical isolate, THSTI-India RPTS 6 Shigella flexneri 2a (IDH-00149) Clinical isolate, THSTI-India RPTS 5 Shigella sonnei (IDH-01529) Clinical isolate, THSTI-India RPTS 4 Shigella sonnei (IDH-01541) Clinical isolate, THSTI-India S9 RPT 76 Staphylococcus aureus ATCC 29213 ATCC, USA RPT 84 Bacillus cereus ATCC 11778 ATCC, USA NKN 8 Listeria monocytogens Lab collection NKN 6 Clostridium perfringens Lab collection NKN 7 Cronobacter sakazakii Lab collection RPT 79 Klebsiella pneumoniae ATCC 600703 ATCC, USA RPT 293 Klebsiella pneumoniae U25 Lab collection NKN 51 Lactobacillus fermentum Lab collection NKN 55 Lactococcus brevis Lab collection NKN 54 Lactobacillus plantarum Lab collection NKN 52 Lactobacillus brevis Lab collection NKN 189 Lactobacillus rhamnosus (L44) Lab collection NKN 147 Lactococcus lactis NZ9000 Lab collection NKN 188 Streptococcus thermophilus NCDC 74 Lab collection Table S5: Plasmids used in the study.
Protein Binding:Article Title: Unveiling the Antibacterial Efficacy of a Benzonitrile Small Molecule, IITR00210, in Shigella Infection.
Article Snippet: .. Table S1: Minimum inhibitory concentration (MIC) of IITR00210 and Kanamycin (control) against various bacterial strains MIC (μg/ml) Strains (Gram-negative) IITR00210 Kanamycin Escherichia coli ATCC 25922 4 2 Escherichia coli MG1655 8 2 Escherichia coli BW25113 16 2 Salmonella enteritidis 1-2 1 Salmonella typhi (NKN 45) 2 2 Salmonella typhimurium (NKN 12) 4 1 Salmonella Vi positive (NKN 10) 2 2 Salmonella enterica ATCC 10708 (RPT 81) 4 1 Salmonella typhi sero enterica (NKN 5) 1 0.5 Shigella flexneri ATCC 9199 (RPT 80) 2 1 Shigella wild type (NKN 47) 2 4 Vibrio cholerae (NKN 43) 4 8 Cronobacter sakazakii (NKN 7) 4 2 Acinetobacter baumannii ATCC 17978 4 2 Acinetobacter baumannii SDF 4 1 Acinetobacter baumannii MTCC 1425 4 2 Gram-negative clinical isolates S6 Shigella boydii (IDH-00007) 4 4 Shigella boydii (IDH-00148) 8 4 Shigella flexneri 2a (IDH-00009) 4 4 Shigella flexneri 2a (IDH-00149) 4 4 Shigella sonnei (IDH-01529) 4 2 Shigella sonnei (IDH-01541) 4 2 Escherichia coli O157:H7 4 2 Klebsiella pneumoniae ATCC 700603 32 32 Klebsiella pneumoniae U25 MDR 16 64 Acinetobacter baumannii AYE MDR 64 >128 Gram-positive strains Bacillus cereus ATCC 11778 16 2 Staphylococcus aureus ATCC 29213 32 1 Clostridium perfringens (NKN 6) 4 >128 Listeria monocytogenes (NKN 8) 4 >64 Lactobacillus brevis (NKN 55) 256 0.5 Lactobacillus brevis (NKN 52) >256 4 Streptococcus thermophilus 128 32 S7 Table S2: Solubility assay of IITR00210 IITR00210 (2—nitro- benzonitrile)Assay parameters Working stock Assay medium % Solubility Kinetic Solubility 160 μM in DMSO 100 mM PBS (pH 7.4) 99 Thermodynamic solubility 13.5 mM in PBS (pH 7.4) 100 mM PBS (pH 7.4) 30 Table S3: Plasma protein binding of IITR00210 IITR00210 Assay parameters Working stock Assay medium Percentage Recovery of compound Plasma protein binding 3.2 mM in DMSO 50% human blood plasma + 1X PBS (pH7.4) 90 Table S4: Strains used in the study. .. Lab strains Strain/Relevant genotype Source/Reference RPT 67 Escherichia coli ATCC 25922 ATCC, USA RPT 26 Escherichia coli MG1655 Lab collection RPT 315 Escherichia coli BW 25113 Lab collection S8 NKN 4 Escherichia coli O157:H7 Lab collection RPT 71 Acinetobacter baumannii ATCC 17978 ATCC, USA RPT 72 Acinetobacter baumannii SDF (ATCC BAA-1709) Lab collection RPT 57 Acinetobacter baumannii MTCC 1425 MTCC, IMTECH-Chandigarh RPT 73 Acinetobacter baumannii AYE (ATCC BAA-1710) ATCC, USA RPT 80 Shigella flexneri ATCC 9199 ATCC, USA NKN 47 Shigella wild type Lab collection RPT 81 Salmonella enterica ATCC 10708 ATCC, USA NKN 5 Salmonella enterica Serovar Typhimurium Lab collection NKN 45 Salmonella typhi Lab collection NKN 12 Salmonella typhimurium Lab collection NKN 10 Salmonella Vi positive Lab collection NASF-sal2 Salmonella enteritidis Lab collection NKN 43 Vibrio cholerae Lab collection RPTS 3 Shigella boydii (IDH-00007) Clinical isolate, THSTI-India RPTS 2 Shigella boydii (IDH-00148) Clinical isolate, THSTI-India RPTS 1 Shigella flexneri 2a (IDH-00009) Clinical isolate, THSTI-India RPTS 6 Shigella flexneri 2a (IDH-00149) Clinical isolate, THSTI-India RPTS 5 Shigella sonnei (IDH-01529) Clinical isolate, THSTI-India RPTS 4 Shigella sonnei (IDH-01541) Clinical isolate, THSTI-India S9 RPT 76 Staphylococcus aureus ATCC 29213 ATCC, USA RPT 84 Bacillus cereus ATCC 11778 ATCC, USA NKN 8 Listeria monocytogens Lab collection NKN 6 Clostridium perfringens Lab collection NKN 7 Cronobacter sakazakii Lab collection RPT 79 Klebsiella pneumoniae ATCC 600703 ATCC, USA RPT 293 Klebsiella pneumoniae U25 Lab collection NKN 51 Lactobacillus fermentum Lab collection NKN 55 Lactococcus brevis Lab collection NKN 54 Lactobacillus plantarum Lab collection NKN 52 Lactobacillus brevis Lab collection NKN 189 Lactobacillus rhamnosus (L44) Lab collection NKN 147 Lactococcus lactis NZ9000 Lab collection NKN 188 Streptococcus thermophilus NCDC 74 Lab collection Table S5: Plasmids used in the study.
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